Tutorials |
· Transcription |
· Splicing |
· Translation |
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Tutorials |
DNA Sequencing |
· Nucleotide addition |
· Inhibition by dideoxynucleotides |
· Animation of DNA sequencing |
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Southern blot |
· Restriction digest of total DNA |
· Transfer of fragments to nitrocellulose (video) |
· Radioactive probe |
· Autoradiogram |
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PCR |
· PCR animation |
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1. Cloning Hemoglobin gene from patient |
· Splicing defect indicated |
· Wild type gene available |
· Choice of insert, vector, method of identification |
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2. Cloning DNA polymerase gene |
· Given pure protein |
· Choice of insert, vector, method of identification |
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3. Cloning Polydactyly gene |
· Given linked RFLP |
· Choice of insert, vector, method of identification |