Molecular Biology
Gene expression:
 
 
 
 
Gene Cloning:
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Problems:
 
 
 
 
 
 
 
 
 
Tutorials
· Transcription
· Splicing
· Translation
 
Tutorials
DNA Sequencing
· Nucleotide addition
· Inhibition by dideoxynucleotides
· Animation of DNA sequencing
 
Southern blot
· Restriction digest of total DNA
· Transfer of fragments to nitrocellulose (video)
· Radioactive probe
· Autoradiogram
 
PCR
· PCR animation
 
1. Cloning Hemoglobin gene from patient
· Splicing defect indicated
· Wild type gene available
· Choice of insert, vector, method of identification
 
2. Cloning DNA polymerase gene
· Given pure protein
· Choice of insert, vector, method of identification
 
3. Cloning Polydactyly gene
· Given linked RFLP
· Choice of insert, vector, method of identification